Vol 7, No 8 (2016) > Chemical Engineering >

Preservation of Rhizopus in Bentonite Clay as Bio-starter

Asri Peni Wulandari, Fadilatul Laela Insan, Tatang Wahyudi, Rezky Iriansyah Anugrah, Ida Indrawati

 

Abstract: Effective and efficient
preservation process is necessary in terms of increasing the fungal usage for
industrial scale as biostarter. The
objective of this study was to identify bentonite
characteristic to be carrier to preserve of Rhizopus
spore and to determine its viability after preservation process. The clay of bentonite
characteristics were identified by BET (Brunaeur Emmett Teller) and SEM-EDS (Scanning Electron Microscopy-Energy
Dispersive Spectroscopy
) for
determining surface properties and elements within the minerals, XRD (X-Ray Diffraction) for identifying the
mineral, and AAS (Atomic Absorption
Spectroscopy
) for determining chemical composition. The growth of
microbial preserved in bentonite tablet after stored for 20, 40, and 60 days
was identified by TPC (Total Plate Count). Bentonite has a main component as silica-SiO2 dan montmorillonit with some
elements existence of magnesium
(Mg), iron (Fe), aluminum (Al), and silica (Si), and Sodium (Na). The spores after preserved need two days longer to grow
back into the mycelium. Viability the spore after storage for 60 days
could be revived 3.0´1010 CFU/g. The results suggest that bentonite could be used as
carrier for the spore of Rhizopus.
Keywords: Bentonite; Carrier; Preservation; Rhizopus; Spore

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