Vol 6, No 7 (2015) > Chemical Engineering >

Effect of Aeration and Nutrients on Saccharomyces cerevisiae Cultivation using Lignocellulosic Hydrolysate from Empty Fruit Bunch

Heri Hermansyah, Adinda Putri Wisman, Diki Firdaus, Rita Arbianti, Tania Surya Utami, Annisa Kurnia



Indonesia has wide palm
oil plantation which produce Empty Fruit Bunch (EFB) waste around 32 million
tons per year. EFB is a potential material for bioethanol through pretreatment,
saccharification, and fermentation. Fermentation has important role in
bioethanol production because this process will convert glucose into
ethanol.  The most common microorganism
used in fermentation process is
Saccharomyces cerevisiae
. But, the use of S. cerevisiae in bioethanol fermentation using lignocellulosic
hydrolysate have a problem that microorganisms cannot grow well. This is due to
the presence of inhibitor in the hydrolysate. Solution for this problem is
using S. cerevisiae which cultivated
on hydrolysate media that will be used in the fermentation (in this case EFB).
This research will investigate cultivation of S. cerevisiae on EFB hydrolysate, to obtain the optimum operating
conditions such as aeration and nutrients. Fed-batch system is used for
cultivation. Optimum condition are determined after analyzing cell number and
ethanol yield from dried S. cerevisiae.
Optimum condition for cultivation are 1 v/v per min aeration and glucose 5 g/L
which produce ethanol yield 24%. We also scale-up the dried yeast into 43.7 g
and need a cost Rp 19,958/g which is more expensive than commercial yeast.

Keywords: Bioethanol; EFB; Hydrolysate; Saccharomyces cerevisiae cultivation

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Alkasrawi, M., Rudolf, A., Liden, G., Zacchi, G., 2006. Influence of Strain and Cultivation Procedure on the Performance of Simultaneous Saccharification and Fermentation of Steam Pretreated Spruce. Enzyme Microbial Technol., Volume 38, pp. 279–286

Direktorat Jendral Perkebunan, (2013),

Lin, Y., Tanaka, S., 2006. Ethanol Fermentation from Biomass Resources: Current State and Prospects. Applied Microbiol Biotechnol., Volume 68, pp. 627–642

Logothetis, S., Walker, G., Nerantzis, E.T., 2007. Effect of Salt Hyperosmotic Stress on Yeast Cell Viability. Natural and Science, Volume 113, pp. 271–284

Nilsson, A., Taherzadeh, M.J., Liden, G., 2001. Use of Dynamic Step Response for Control of Fed-batch Conversion of Lignocellulosic Hydrolyzates to Ethanol. J Biotechnol., Volume 89, pp. 41–53

Piarpuzan, D., Quintero, J.A., Cardona, C.A., 2011. Empty Fruit Bunches from Oil Palm as Potential Raw Material for Fuel Ethanol Production. Biomass and Bioenergy, Volume 35, pp. 1130–1137

Petersson, A., Liden, G., 2007. Fed-Batch Cultivation of Saccharomyces cerevisiae on Lignocellulosic Hydrolyzate. Biotechnol Lett., Volume 29, pp. 219–225

Sherman, F., 2001. Getting Started with Yeast. Methods Enzymol, Volume 350, pp. 3–41

Tanguay, A.E., Bogert, A.B., 1974. Survival of Saccharomyces cerevisiae and Sarcina lutea at Refrigerator Temperatures. Applied Microbiology, Volume 27(6), pp. 1175–1176